Conor Benn and Chris Eubank Jr will be kept apart at Thursday news conference after a volatile face-off on Tuesday.
Eubank smashed an egg across the face of Benn, sparking a melee on stage and a furious reaction from Benn's father Nigel and promoter Eddie Hearn.
Benn is due to fight Eubank on 26 April and the bitter rivals will meet again at another news conference on Thursday at Tottenham Hotspur Stadium, where the fight will take place.
Thirty-five-year-old Eubank will be favourite against 28-year-old Benn, who steps up from the welterweight limit of 10st 7lb (147lb) for the 11st 6lb (160lb) middleweight contest.
"It was always going to be a volatile build-up," Hearn told BBC Sport.
"All bets are off now and we've got to be careful moving forward, particularly on Thursday at Spurs. We need to make sure we keep them apart.
"These people, you don't forget that. It's not a case of sleep on it and calm down. We need to keep them apart because we won't get to April."
Warring fighters have been separated at media events before. Tyson Fury and Deontay Wilder were confined to opposite ends of the stage at their final weigh-in before their trilogy fight in 2021.
Hearn was filmed shoving Eubank's promoter Ben Shalom during the melee on Tuesday, but Shalom said he had no idea his fighter planned to slap Benn with an egg.
Security rushed in to stop the situation from escalating further after a news conference that was full of insults about family rifts and failed drug tests.
The boxers continue their family rivalry after their fathers - Chris Eubank Sr and Nigel Benn - had two fights in the 1990s but a scheduled contest between the sons in October 2022 was called off after Benn failed a voluntary drugs test, prompting a two-year battle with anti-doping authorities, before his suspension was lifted in November.
Benn denied intentionally doping, but a report from sanctioning body the WBC suggested an overconsumption of eggs could have caused Benn's two failed drug tests.
Hearn expects the British Boxing Board of Control to speak to Eubank about his conduct and BBC Sport has contacted the Board for comment.
Steve Bunce, speaking on his 5 Live Boxing podcast, said there would definitely be "fine" for Eubank's conduct.
"The premediated nature of the attack is not what I was a fan of," Hearn said.
"We've done it before. Whether it's Haye-Bellew, very volatile fights in the past. We keep them away.
"We can't afford to keep them in distance of each other. Because as I say, it is attack on sight mentality [now for Benn]."
"I understand things can spill over," Hearn added.
"But now we've just got to make sure they're kept apart.
"We've got to be careful. But at the same time we've got to continue the build-up, have the press conference at Spurs and make sure camps are aware we do have a code of conduct we need to follow."